Siglec-2/CD22 Antibody (MYG13), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2446020.02MG

Catalog No. NBP24460200

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence

Siglec-2/CD22 Monoclonal specifically detects Siglec-2/CD22 in Human, Mouse, Rat samples. It is validated for Flow Cytometry.

Specifications

• Antigen: Siglec-2/CD22
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Alias: B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule, CD22 antigenMGC130020, CD22 molecule, sialic acid binding Ig-like lectin 2, Sialic acid-binding Ig-like lectin 2, SIGLEC-2, SIGLEC2FLJ22814, T-cell surface antigen Leu-14
• Host Species: Mouse
• Molecular Weight of Antigen: 135 kDa
• Quantity: 0.02 mg
• Research Discipline: Adaptive Immunity, DNA Repair, Immunology
• Gene ID (Entrez): 933
• Target Species: Human, Mouse, Rat
• Form: Purified

• Applications: Flow Cytometry, SDS-Page, Immunofluorescence
• Clone: MYG13
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Symbols: CD22
• Immunogen: Human Raji cells
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

For Research Use Only