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Siglec-2/CD22 Antibody (MYG13), Novus Biologicals™
SDP

Catalog No. NBP24460200 Shop All R&D Systems Products
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NBP24460200 0.02 mg
NBP24460201 0.1 mg
NBP24460202 0.2 mg
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Catalog No. NBP24460200 Supplier Novus Biologicals Supplier No. NBP2446020.02MG
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Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence

Siglec-2/CD22 Monoclonal specifically detects Siglec-2/CD22 in Human, Mouse, Rat samples. It is validated for Flow Cytometry.

Specifications

Antigen Siglec-2/CD22
Applications Flow Cytometry, SDS-Page, Immunofluorescence
Classification Monoclonal
Clone MYG13
Concentration 0.2 mg/mL
Conjugate Unconjugated
Dilution Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
Formulation 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule, CD22 antigenMGC130020, CD22 molecule, sialic acid binding Ig-like lectin 2, Sialic acid-binding Ig-like lectin 2, SIGLEC-2, SIGLEC2FLJ22814, T-cell surface antigen Leu-14
Gene Symbols CD22
Host Species Mouse
Immunogen Human Raji cells
Molecular Weight of Antigen 135 kDa
Purification Method Protein A or G purified
Quantity 0.02 mg
Regulatory Status RUO
Research Discipline Adaptive Immunity, DNA Repair, Immunology
Primary or Secondary Primary
Gene ID (Entrez) 933
Test Specificity Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
Target Species Human, Mouse, Rat
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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For Research Use Only

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