SIRPA Monoclonal Antibody (OTI1C6), TrueMAB™, OriGene

Mouse Monoclonal Antibody

Supplier:  OriGene TA507333

Catalog No. 50-167-7066

Description

SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and hematopoietic stem cells. SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. The ligand binding of SIRP alpha to integrin-associated protein CD47 results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of SIRP alpha, which mediates the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. Ligation of SIRP alpha with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs. SIRP alpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion, and plays a general role in different physiological and pathological processes. Cancer cells highly express CD47, which activates SIRP alpha and inhibits macrophage-mediated destruction of cancerous cell growth.

Specifications

• Antigen: SIRPA
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 50% glycerol and 0.02% sodium azide
• Gene Accession No.: P78324
• Gene Symbols: SIRPA
• Immunogen: Full length human recombit protein of human SIRPA produced in HEK293T cell.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: Western Blot
• Clone: OTI1C6
• Conjugate: Unconjugated
• Gene: SIRPA
• Gene Alias: BIT, CD172A, MFR, MYD-1, P84, PTPNS1, SHPS1, SIRP
• Host Species: Mouse
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 140885
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid