Smad2, p Ser465, p Ser467 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody

Supplier:  Novus Biologicals NBP244217

Catalog No. NBP244217

Description

Specifically detects Smad2/3 in Human samples, and it is validated for ELISA, Western Blotting

Smad2 Polyclonal specifically detects Smad2 in Human samples. It is validated for Western Blot, ELISA.

Specifications

• Antigen: Smad2 (p Ser465, p Ser467)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Alias: hMAD-2, hSMAD2, JV18, JV18-1SMAD, mothers against DPP homolog 2 (Drosophila), MAD homolog 2, Mad protein homolog, Mad, mothers against decapentaplegic homolog 2, MADH2MGC34440, MADR2MGC22139, Mad-related protein 2, mother against DPP homolog 2, mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Sma- and Mad-related protein 2, SMAD 2, SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila), SMAD, mothers against DPP homolog 2, Smad2
• Host Species: Rabbit
• Molecular Weight of Antigen: 52.31 kDa
• Quantity: 0.1 mg
• Research Discipline: Cancer, Cell Cycle and Replication, Stem Cell Signaling Pathway, Wnt Signaling Pathway
• Gene ID (Entrez): 4087
• Target Species: Human, Mouse, Rat, Bovine, Orangutan
• Isotype: IgG

• Applications: Western Blot, ELISA
• Concentration: 0.94 mg/mL
• Dilution: Western Blot 1:1000-1:3000, ELISA 1:10000
• Gene Accession No.: Q15796
• Gene Symbols: SMAD2
• Immunogen: Smad2 [p Ser465, p Ser467] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal of human Smad2 protein. (Uniprot: Q15796)
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Anti-Smad2 pS465 pS467 antibody is directed against the phosphorylated form of human Smad2 protein at the pS465 and pS467 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad2. Reactivity with non-phosphorylated human Smad2 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross-reactivity with Smad2 protein from human, mouse, rat, orangutan, and bovine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

For Research Use Only