Smad3, p Ser423, p Ser425 Antibody, DyLight 550, Novus Biologicals™

Rabbit Polyclonal Antibody

Supplier:  Novus Biologicals NBP177836R

Catalog No. NBP177836R

Description

Smad3 (p Ser423) Polyclonal antibody specifically detects Smad3 (p Ser423) in Human, Mouse samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin).

Specifications

• Antigen: Smad3 (p Ser423)
• Classification: Polyclonal
• Dilution: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin
• Gene Alias: DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Research Discipline: Phospho Specific, Signal Transduction, Wnt Signaling Pathway
• Gene ID (Entrez): 4088
• Target Species: Human, Mouse, Rat, Pig, Bovine, Chicken, Xenopus species, Zebrafish
• Isotype: IgG

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
• Conjugate: DyLight 550
• Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
• Gene Symbols: SMAD3
• Immunogen: Smad3 [p Ser423, p Ser425] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. (Uniprot: P84022)
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Test Specificity: This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus species laevis, Xenopus species tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
• Content And Storage: Store at 4C in the dark.