Smad3, p Ser423, p Ser425 Antibody, Novus Biologicals™

Catalog No.	Quantity
NBP177836SS	0.025 mg

Catalog No. NBP177836SS Supplier Novus Biologicals Supplier No. NBP177836SS

Description

Rabbit Polyclonal Antibody

Smad3 (p Ser423, p Ser425) Polyclonal antibody specifically detects Smad3 (p Ser423, p Ser425) in Human, Mouse samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Single Cell Western.

Specifications

• Antigen: Smad3 (p Ser423, p Ser425)
• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Concentration: 1.1 mg/ml
• Conjugate: Unconjugated
• Dilution: Western Blot 1:2000-1:20000, Flow Cytometry 1:10-1:1000, ELISA 1:15000-1:30000, Immunohistochemistry 1:500, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:10-1:500, Single Cell Western 100 ug/ml
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Accession No.: NC_000015.9
• Gene Alias: DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), Mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, pSMAD, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
• Gene Symbols: SMAD3
• Host Species: Rabbit
• Immunogen: Smad3 [p Ser423, p Ser425] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. (Uniprot: P84022)
• Molecular Weight of Antigen: 48.08 kDa
• Purification Method: Affinity Purified
• Quantity: 0.025 mg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 4088
• Test Specificity: This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus species laevis, Xenopus species tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
• Target Species: Human, Mouse, Rat, Pig, Bovine, Chicken, Xenopus species, Zebrafish
• Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
• Isotype: IgG