Thermo Scientific™ Pierce™ SMCC

Description

No-Weigh™ Format

Thermo Scientific™ Pierce SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane-stabilized spacer arm (8.3 Ås).

Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging helps prevent loss of reagent reactivity and contamination over time by minimizing repetitive opening and closing of vial. The format enables use of fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

• Reactive groups: NHS ester and maleimide
• Reactive toward: amino and sulfhydryl groups
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-SMCC
• High purity, crystalline reagent can be used to create high-purity maleimide-activated derivatives
• Cyclohexane bridge confers added stability to the maleimide group making SMCC an ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C

Applications:

• Enzyme labelling of antibodies - both enzyme and antibody specificity can be preserved
• Create specific bioconjugates via one- or two-step crosslinking reactions
• Create sulfhydryl-reactive, maleimide-activated carrier proteins for coupling haptens

Specifications

• Chemical Reactivity: Amine-Sulfhydryl
• Cleavable: By Thiols
• Molecular Weight (g/mol): 334.32
• PEGylated: No
• Spacer Arm Length: 8.3 Å
• Content And Storage: Upon receipt store desiccated at 4°C.
• Cell Permeability: Yes
• Shipping Condition: Ambient
• Product Line: No-Weigh™, Pierce™
• Labeling Method: Chemical Labeling
• Crosslinker Type: Heterobifunctional
• Reactive Moiety: Maleimide, NHS Ester
• Spacer: Short (<10 Å)
• Form: Powder
• Quantity: 10 x 1 mg
• Solubility: DMF, DMSO
• Format: Standard, Single-use
• Water Soluble: No
• Product Type: Crosslinker

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.