Novus Biologicals™ SP-D Antibody Pair [HRP]

Description

Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Mouse SP-D.

Specifications

• Assay Range: 62.5 pg/mL
• Assay Sensitivity: 62.5-4000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Mouse SP-D.
• Target Species: Mouse
• For Use With (Application): Sandwich ELISA
• Gene Alias: COLEC7collectin-7, collectin 7, Collectin-7, Lung surfactant protein D, PSPD, PSP-D, SFTP4pulmonary surfactant-associated protein D, SP-Dpulmonary surfactant apoprotein, surfactant protein D, surfactant, pulmonary-associated protein D, surfactant-associated protein, pulmonary 4
• Gene ID (Entrez): 6441
• Gene Symbol: SFTPD
• Host Species: Rabbit
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of rabbit anti-SP-D polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 1 mg/mL of rabbit anti-Mouse SP-D monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 105 ng of recombinant Mouse SP-D.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cell Cycle and Replication
• Storage Requirements: Storage is content dependent.
• Target: SP-D
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.6 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.