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Invitrogen™ SSEA3 Monoclonal Antibody (eBioMC-631 (MC-631)), PE, eBioscience™
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Catalog No. p-7178478
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100 Tests
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Catalog No. Quantity
50-112-20 100 Tests
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Catalog No. 50-112-20 Supplier Invitrogen™ Supplier No. 12883342
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Description

Rat Monoclonal Antibody

Description: The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3). Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES) and down regulated following differentiation. Applications Reported: This eBioMC-631 (MC-631) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioMC-631 (MC-631) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
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Specifications

Antigen SSEA3
Applications Flow Cytometry
Classification Monoclonal
Clone eBioMC-631 (MC-631)
Concentration 5 μL/Test
Conjugate PE
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias SSEA-3; stage-specific embryonic antigen 3
Host Species Rat
Purification Method Affinity chromatography
Quantity 100 Tests
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human, Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgM
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Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov
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