Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Description

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Thermo Scientific™ SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems and quantitation. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, enabling multiple film or camera exposures.

SuperSignal West Dura Extended Duration Substrate characteristics include:

• Sensitivity: mid-femtogram
• Stability: 24-hr working solution stability; 1-year kit stability at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 24 hours
• Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)

Specifications

• Product Type: Substrate
• Detection Method: Chemiluminescence
• Form: Liquid
• For Use With (Application): Western Blot
• Membrane Compatibility: Nitrocellulose, PVDF
• Product Line: SuperSignal™
• Quantity: 20 mL
• Substrate: Horseradish Peroxidase
• Recommended Antibody Concentrations: 2°: 1:50-250K (4-20ng/mL), 1°: 1:5K (0.02- 1μg/mL)
• Sensitivity: Mid-femtogram
• Signal Duration: 24 hr.
• Sufficient For: 2 Mini-Gel Blots, 200 sq. cm of Membrane
• Kit Contents: Stable Peroxide Buffer, 10mL, Luminol/Enhancer, 10mL
• Content And Storage: Sufficient For: 2 mini-gel blots; 200 cm² of membrane
  • Luminol/Enhancer, 10 mL
  • Stable Peroxide Buffer, 10 mL
  
  Store at room temperature.

For Research Use Only. Not for use in diagnostic procedures.