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Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate
Description
Thermo Scientific™ SuperSignal West Femto Substrate is an ultra-sensitive enhanced chemiluminescent substrate (ECL substrate) for detection of peroxidase activity from HRP-conjugated antibodies and probes in Western blotting.
Highlights:
ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes
Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes
Quantitative – produces quantitative signal that is measurable over two orders of magnitude
Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks)
Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system
Excellent signal duration – 8 hours of usable light-output when conditions are optimized
Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature

Specifications
Specifications
Product Type | Substrate |
Detection Method | Chemiluminescence |
Form | Liquid |
Product Line | SuperSignal™ |
Quantity | 4 x 100 mL |
Substrate | Horseradish Peroxidase |
Recommended Antibody Concentrations | 2°: 1:100-500 K (2-10 ng/mL) 1°: 1:5 K (10- 200 ng/mL) |
Sensitivity | Low to mid-femtogram |
Signal Duration | 6 to 8 hr. |
Kit Contents | Luminol/Enhancer (50 mL), Stable Peroxide Buffer (50 mL) |
For Research Use Only. Not for use in diagnostic procedures.
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