Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Catalog Number	Mfr. No.	Kit Contents	Quantity
PI34094	Thermo Scientific™ 34094	Stable Peroxide Buffer, 10mL, Luminol/Enhancer, 10mL	20 mL Kit
PI34095	Thermo Scientific™ 34095	Luminol/Enhancer, 50mL, Stable Peroxide Buffer, 50mL	100 mL Kit
PI34096	Thermo Scientific™ 34096	Stable Peroxide Buffer, 100mL, Luminol/Enhancer, 100mL	200 mL Kit

Description

Thermo Scientific™ SuperSignal West Femto Substrate is an ultra-sensitive enhanced chemiluminescent substrate (ECL substrate) for detection of peroxidase activity from HRP-conjugated antibodies and probes in Western blotting.

SuperSignal West Femto Substrate Maximum Sensitivity Substrate is an ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme. The substrate kit enables detection of low femtogram (that's mid-zeptomole!) amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are small to be seen with typical ECL substrates.

Highlights:

• ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes

• Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes

• Quantitative – produces quantitative signal that is measurable over two orders of magnitude

• Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks)

• Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system

• Excellent signal duration – 8 hours of usable light-output when conditions are optimized

• Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature

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