Thermo Scientific™ SurePac™ Oligo RP MDi™ HPLC Columns

Catalog No.	Diameter (Metric)	Length (Metric)
43-712-052132	2.1 mm	50 mm
43-712-022132	2.1 mm	20 mm
43-712-050332	300 μm	50 mm

Catalog No. 43-712-052132 Supplier Thermo Scientific™ Supplier No. 43712052132

Description

SurePac Oligo RP MDI is a robust reversed-phase column designed for oligonucleotide analysis by HPLC and LC-MS. It delivers high recovery, bioinert column hardware, and reproducible separations, enabling reliable profiling of oligonucleotides of any size.

Thermo Scientific™ SurePac™ Oligo RP MDi™ HPLC columns deliver high-resolution, reproducible separations for therapeutic oligonucleotides and nucleic acids across R and D and QC workflows. Built on monodisperse, supermacroporous polymeric packing with bio-inert hardware, they provide stable performance even under elevated pH and temperature conditions, resolving truncation impurities while supporting large oligos and intact nucleic acids for scalable LC-MS and LC-UV analysis.

• Purpose-built for oligonucleotide RP separations
• Optimized for DNA and RNA oligonucleotides, including modified sequences, delivering consistent retention and selectivity across short and long oligos.
• Bioinert, metal-free fluid path
• Reduces metal/oligonucleotide interactions to improve recovery, minimize adsorption, and deliver cleaner peak shapes for sensitive analyses.
• Optimized for ion-pair RP LC-MS
• Fully compatible with common ion-pairing reagents such as HFIP/TEA, enabling sensitive, reproducible LC-MS detection.
• High recovery of long and modified oligonucleotides
• High resolution power for oligonucleotides spanning over a wide size range from small to (ultra) large
• Supports efficient analysis of long, hydrophobic, and chemically complex oligos without compromising signal or resolution.
• Robust stationary phase for demanding methods
• Maintains stable performance under high organic content and elevated temperatures, supporting fast gradients and long column lifetimes.
• Excellent peak shape and impurity resolution
• Delivers sharp, symmetrical peaks and reliable separation of closely related species, including impurities and sequence variants.
• Reproducible, manufacturing-controlled performance
• Ensures consistent results across columns and batches, simplifying method transfer and routine use.
• Flexible for development and routine workflows
• Suitable for method development, impurity profiling, and QC, reducing the need for multiple column chemistries.
• Compatible with UHPLC and HPLC systems
• Designed for seamless integration into modern LC platforms and existing laboratory setups.
• Built for confident bioanalytical results
• Improves robustness, reduces variability, and supports reliable data generation in oligonucleotide workflows.

Specifications

• Column Format: Analytical
• Diameter (Metric): 2.1 mm
• Flow Rate: 0.2 to 0.6 mL/min.
• Length (Metric): 50 mm
• Max. Pressure: 6500 psi (448 bar)
• Particle Size: 2.5 μm
• pH: 2 to 12
• Pore Size: Proprietary supermacroporous
• Temperature: 5°C to 90°C
• Stationary Phase: Poly-DVB
• Packing Material: Polymeric