SWAP-70, Rabbit anti-Human, Polyclonal, Millipore Sigma™

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma ABC168825UG

Catalog No. ABC168825UG

Description

Anti-SWAP-70, Cat. No. ABC1688, is a rabbit polyclonal antibody that detects Switch-associated protein 70 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Switch-associated protein 70 (UniProt: Q9UH65; also known as SWAP-70) is encoded by the SWAP70 (also known as KIAA0640, HSPC321) gene (Gene ID: 23075) in human. SWAP-70 is a phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent guanine nucleotide exchange factor (GEF) that, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. SWAP-70 regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion. It is expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue. In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG, but not IgM. It is also widely expressed in spleen, kidney, lung, and liver. SWAP-70 has an N-terminal putative EF-hand domain, a pleckstrin homology (PH) domain (aa 210-306) that binds phosphatidylinositol 3,4,5-trisphosphate and is responsible for membrane localization, a tri-partite coiled-coil region (aa 316-539), and a C-terminal F-actin-binding site through which it specifically binds non-muscle actin. (Ref.: Chacón-Martínez, CA et al. (2103). J. Biol. Chem. 288(40); 28687-28703).

Specifications

• Antigen: SWAP-70
• Classification: Polyclonal
• Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected SWAP-70 in human tonsil and human lung tissue sections.
• Gene Alias: Switch-associated protein 70
• Host Species: Rabbit
• Molecular Weight of Antigen: ∽70 kDa observed; 69.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Quantity: 25 μg
• Research Discipline: Apoptosis & Cancer
• Gene ID (Entrez): NP_001284643
• Target Species: Human

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Conjugate: Unconjugated
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Symbols: SWAP70;KIAA0640;HSPC321
• Immunogen: KLH-conjugated linear peptide corresponding to 12 amino acids from the N-terminal region of human Switch-associated protein 70.
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This rabbit polyclonal antibody detects human Switch-associated protein 70. It targets an epitope with in 12 amino acids from the N-terminal region.
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.