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Invitrogen™ Tango™ OPRK1-bla U2OS Cells

Catalog No. K1576
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2 x 106 cells
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K1576 2 x 106 cells
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Catalog No. K1576 Supplier Invitrogen™ Supplier No. K1576
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Description

Tango™ OPRK1-bla U2OS Cells

The Tango™ OPRK1-bla U2OS cells contain the human Opiod Receptor Kappa 1 (OPRK1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ OPRK1-bla U2OS cells cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ OPRK1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_000912
Assay Entry Cell-based beta-arrestin recruitment
Cell State Dividing Cells
Content And Storage K1576 Tango™ OPRK1-bla U2OS cells
(LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• Tango™ OPRK1-bla U2OS cells
• Tango™ OPRK1-bla U2OS Assay Protocol
• Certificate of Analysis

Storage:
The Tango™ OPRK1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cytoplasmic Tail Tail from AVPR2 GPCR
Detection Method Fluorescence
Gene Symbol OPRK1
Population Status Pool of Stable Cells
Product Line Tango
Quantity 2 x 106 cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry OPRK1
Type U2OS Cell
Cell Line U2OS
System Type Tango™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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