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Thermo Scientific™ Tango™ OPRL1-bla U2OS Cells
Description
The Tango™ OPRL1-bla U2OS cells contain the human Opioid Receptor-Like 1 (OPRL1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ OPRL1-bla U2OS cells have been functionally validated for Z'-factor and EC50 concentrations of established ligands. In addition, Tango™ OPRL1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.
Specifications
Specifications
| Accession Number | NM_000913 |
| Assay Entry | Cell-based beta-arrestin recruitment |
| Cell State | Dividing Cells |
| Content And Storage | LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • Tango™ OPRL1-bla U2OS cells • Tango™ OPRL1-bla U2OS Assay Protocol • Certificate of Analysis The Tango™ OPRL1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
| Cytoplasmic Tail | Tail from AVPR2 GPCR |
| Detection Method | Fluorescence |
| For Use With (Application) | FRET |
| Gene Symbol | OPRL1 |
| Human Protein Class | G-Protein Coupled Receptor (GPCR) |
| Population Status | Single Cell Clone Origin |
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For Research Use Only. Not for use in diagnostic procedures.
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