TCR V beta 5 Monoclonal Antibody (MEM-262), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA119020

Catalog No. 01-674-993

Description

This antibody recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V (beta5.3)-specific antibodies. Functional Application: Activation.

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

Specifications

• Antigen: TCR V beta 5
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.4
• Gene Accession No.: 0
• Gene Symbols: TRB
• Immunogen: Human thymoma cell line HPB-ALL.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Clone: MEM-262
• Conjugate: Unconjugated
• Gene: TRB
• Gene Alias: T cell receptor beta locus; TCR V beta5; TCRB; TCRV b5; TCRV beta 5; TRB; TRB@; Vb5; Vbeta5
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 6957
• Content And Storage: 4° C, do not freeze
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov