Cytiva TempliPhi™ Sequence Resolver Kit

Description

Includes

Sample buffer, Reaction buffer and Enzyme mix

Uses modified nucleotides in isothermal rolling circle amplification (RCA) to improve results with difficult to sequence templates

• Cultureless preparation from bacterial colonies, glycerol stock or purified DNA, saves time and reagents
• Requires 20 minutes hands-on time
• Excellent sequence finishing performance resolving sequencing stop and compression issues associated with difficult templates
• Improve difficult template sequencing success with up to 820 bp Phred 20 read length
• For use directly in cycle sequencing reactions without purification
• Available in sizes of 20, 50 and 500 reactions

Specifications

• Content And Storage: Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°CThaw components on ice and maintain at 0°C to 4°C during handling
• Description: illustra TempliPhi Sequence Resolver Kit uses the highly processive, strand-displacing Phi29 DNA polymerase and modified nucleotides to amplify circular templates for subsequent sequencing.
• For Use With (Application): Amplification of difficult templates for successful DNA sequencing
• Includes: 1 x 90μL Sample Buffer, 1 x 90μL Reaction Buffer, 1 x 90μL Enzyme mix
• No. of Reactions: 20 reactions
• Product Type: illustra TempliPhi Sequence Resolver Kit
• Test Time: 18 h