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TGF alpha Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Rabbit Polyclonal Antibody
$250.00 - $2175.00
Specifications
Antigen | TGF alpha |
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Concentration | 0.1-1.0 mg/mL |
Applications | ELISA, Western Blot |
Classification | Polyclonal |
Conjugate | Biotin |
Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
500P16BT25
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Invitrogen™
500P16BT25UG |
25 μg |
Each for $250.00
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500P16BT50
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Invitrogen™
500P16BT50UG |
50 μg |
Each for $319.00
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50-271-8644
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Invitrogen™
500P16BT500UG |
500 μg |
Each for $2,175.00
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Description
AA Sequence of recombinant protein: VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TGF-alpha. Anti-Human TGF-alpha-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human TGF-alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TGF-alpha (500-P16) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TGF-alpha. Western Blot: To detect Human TGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TGF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P16BT-1MG will be provided as 2 x 500 μg
TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.Specifications
TGF alpha | |
ELISA, Western Blot | |
Biotin | |
Rabbit | |
Human | |
P01135 | |
7039 | |
E.coli-derived, 5.5 kDa Recombinant Human TGF-alpha. | |
Antigen affinity chromatography | |
TGFA |
0.1-1.0 mg/mL | |
Polyclonal | |
Lyophilized | |
RUO | |
PBS with no preservative | |
EGF-like TGF; ETGF; I79_019067; LOC100009150; Protransforming growth factor alpha; RATTGFAA; TFGA; TGF a; TGF type 1; TGF a; TGFA; TGFAA; TGFalpha; TGF-alpha; TGFa; Transforming growth factor; Transforming growth factor alpha; transforming growth factor, alpha; transforming growth factor-alpha; wa1; wa-1; waved 1 | |
TGFA | |
Primary | |
-20°C |
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