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Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, Fluorescein

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Manufacturer:  Thermo Scientific™ K0984

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Catalog No. FERK0984


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Includes:
  • Luminaris HiGreen Fluorescein qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer. The master mix contains fluorescein passive reference dye for specific real-time platfo

Description

Description

  • UDG in the master mix to prevent carry-over contamination
  • Equal performance of blue and colorless master mixes
  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols
  • Excellent qPCR performance
  • Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity: single copy detection
  • Wide linear range: accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience: ready-to-use 2X master mix
Specifications

Specifications

5000 x 20μL rxns
Store at -20°C.
Detection Method: SYBR, PCR Method: qPCR
BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5
5000 Reactions
DNA, dsDNA
2X
Real time PCR (qPCR), gene expression analysis, siRNA validation, genotyping and pathogen detection
Luminaris HiGreen Fluorescein qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains fluorescein passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied nuclease-free water.
Safety and Handling

Safety and Handling

ShelfLife : 2 Years

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