Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Amplify DNA directly from variety of human and animal samples with no prior purification with Phire Tissue Direct PCR Master Mix.

Catalog Number	Mfr. No.	Quantity
F170S	Thermo Scientific™ F170S	250 x 20 μL Reactions
F170L	Thermo Scientific™ F170L	1250 x 20 μL Reactions

Description

The Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle). The kit also includes Thermo Scientific DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.

Highlights

• Direct PCR – sample is added directly to PCR reaction, therefore therese is no need for time-consuming and expensive DNA purification steps
• Dilution & Storage protocol available – allows tens of PCR reactions from one tiny sample and allows re-testing
• Specially engineered Phire DNA polymerase – extremely short PCR protocol times
• Master Mix format with premixed gel loading dye – minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

Includes

• 2X Phire Tissue Direct PCR Master Mix
• Reaction voume with dilution & storage protocol: 20 μL
• Reaction voume with direct protocol: 50 μL
• Dilution Buffer
• DNARelease Additive
• Universal control primer mix (25 μM each)
• Water, nuclease- free
• O'GeneRuler Express DNA Ladder

Specifications

• GC-Rich PCR Performance: Medium
• Polymerase: Phire Hot Start II DNA polymerase
• Includes Label or Dye: Yes
• Product Type: Direct PCR Master Mix
• Hot Start: Built-In Hot Start
• Reaction Format: SuperMix or Master Mix
• Starting Material: DNA

• PCR Method: Direct PCR
• Reaction Speed: Fast
• Product Line: Phire
• For Use With (Application): Genotyping
• Overhang: Blunt
• Sample Type: Tissue

For Research Use Only. Not for use in diagnostic procedures.