Thermo Scientific™ Phusion U Hot Start DNA Polymerases

Description

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Thermo Scientific Phusion U DNA Polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion polymerase, Phusion U polymerase overcomes an important limitation of proofreading enzymes: it is able to incorporate dUTP and read through uracil present in DNA templates.

In addition to the uracil usage possibility, Phusion U polymerase features all the superior properties of other Phusion DNA polymerases: great accuracy, speed, ability to amplify long amplicons up to 20 kb, and a high specificity with Affibody-based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.

Phusion U Hot Start Green DNA Polymerase is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U polymerase performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.

Highlights

• Accuracy—high fidelity DNA polymerase (25X Taq)
• Uracil-tolerance—engineered to incorporate dUTP and amplify uracil containing templates
• Specificity—hot start for reduced nonspecific amplification and primer degradation
• Speed—short extension times (15–30 s/kb)
• Green format—permits direct loading of PCR products on gels

Applications

• Amplification of bisulphite-converted DNA
• Amplification of damaged or aged DNA
• Carry-over contamination control
• Uracil-excision based (USER) cloning methods

Using Phusion DNA polymerases

Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

Specifications

• Concentration: 2 U/μL
• Content And Storage: • Phusion U Hot Start DNA Polymerase, 2 U/μL
  • 5X Phusion Green HF & GC Buffers
  • DMSO
  • 50 mM MgCl₂ solution
  
  Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl₂ in the final 1X concentration.
  Store at -20°C.
• GC-Rich PCR Performance: High
• Polymerase: Phusion U Hot Start DNA Polymerase
• Reaction Speed: Fast
• Product Type: Hot Start DNA Polymerase
• Quantity: 100 units
• Shipping Condition: Dry Ice
• For Use With (Application): Hot-start PCR, High-fidelity PCR
• Fidelity (vs. Taq): 25X
• Hot Start: Built-In Hot Start
• No. of Reactions: 100 Reactions
• Overhang: Blunt
• Reaction Format: Standalone
• Size (Final Product): 20 kb or less
• Color: Green

For Research Use Only. Not for use in diagnostic procedures.