Thermo Scientific™ Phusion U Hot Start PCR Master Mix

Description

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Thermo Scientific Phusion U Hot Start PCR Master Mix is a convenient 2X formula that contains Phusion U Hot Start DNA Polymerase, nucleotides, and an optimized reaction buffer including MgCl₂.

Phusion U DNA Polymerase is a novel engineered high-fidelity enzyme developed using fusion technology. Designed with a proprietary mutation in the dUTP binding pocket of Phusion polymerase, Phusion U polymerase is able to overcome important limitations of proofreading enzymes to incorporate dUTP and read through uracil present in DNA templates.

Phusion U polymerase features properties of Phusion DNA polymerases and offers the ability to amplify long amplicons up to 20 kb and a high specificity with affibody based hot start.

These features make Phusion U Hot Start DNA Polymerase an excellent choice for such applications as amplification of bisulfite-converted or damaged DNA as well as use of carryover contamination control.

Highlights

• High-fidelity DNA polymerase (25X Taq)
• Engineered to incorporate dUTP and amplify uracil containing templates
• Hot start for reduced nonspecific amplification and primer degradation
• Only template and primers need to be added for use
• Short extension times (15–30 s/kb)

Applications

• Amplification of bisulfite-converted DNA
• Amplification of damaged or aged DNA
• Carry-over contamination control
• Uracil-excision based (USER) cloning methods

Using Phusion DNA Polymerases

• Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

Specifications

• Concentration: 2X
• Content And Storage: • Phusion U Hot Start DNA Polymerase
  • 2X reaction buffer
  • 3 mM MgCl₂
  • 400 μM each of dATP, dTTP, dCTP and dGTP
  
  Master mix is supplied with nuclease-free water.
  Store at -20°C.
• GC-Rich PCR Performance: Medium
• Polymerase: Phusion U Hot Start DNA Polymerase
• Reaction Speed: Fast
• Product Type: Hot Start PCR Master Mix
• Quantity: 500 reactions
• Shipping Condition: Dry Ice
• For Use With (Application): Hot-start PCR
• Fidelity (vs. Taq): 25X
• Hot Start: Built-In Hot Start
• No. of Reactions: 500 Reactions
• Overhang: Blunt
• Reaction Format: SuperMix or Master Mix
• Size (Final Product): 20 kb or less

For Research Use Only. Not for use in diagnostic procedures.