Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ RevertAid™ Premium Double Stranded cDNA Synthesis Kit

The Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA.

Manufacturer:  Thermo Scientific™ K2561

Catalog No. FERK2561


Add to cart

Includes: First Strand Enzyme Mix, 4X First Strand Reaction Mix, Second Strand Enzyme Mix, 5X Second Strand Reaction Mix, 0.5M EDTA ( pH 8.0), RNase I (10 u/µL), Control RNA (0.5µg/µL), Oligo(dT)18 Primer, 100µM, 0.5µg/µL, Random Hexamer Primer, 100µM, 0.2µg/µL,Water, nuclease-free, Detailed Protocol

Description

Description

The RevertAid™ Premium Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA. First- and second-strand cDNA synthesis reactions are performed in the same tube without the need for intermediate organic extraction or ethanol precipitation steps. This convenient one-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

The Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA. First- and second-strand cDNA synthesis reactions are performed in the same tube without the need for intermediate organic extraction or ethanol precipitation steps. This convenient one-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
Highlights
· Efficient synthesis of full length double-stranded cDNA
· Fast - procedure completed in less than 2 hours
· Convenient - premixed components
· Complete - includes all primers, controls and residual RNA removal reagents
Applications
· Full-length double-stranded blunt-end cDNA synthesis for cloning
· Double-stranded cDNA library construction
Includes
· First Strand Enzyme Mix
· 4X First Strand Reaction Mix
· Second Strand Enzyme Mix
· 5X Second Strand Reaction Mix
· 0.5 M EDTA, pH U/µL
· Control RNA, 0.5 µg/µL
· RNase I, 10 U/µL
· Control RNA, 0.5 µg/µL
·Oligo(dT)18 Primer, 100 µM, 0.5 µg/µL
·Random Hexamer Primer, 100 µM, 0.2 µg/µL
·Water, nuclease-free
· Detailed Protocol

Hazardous:No
> Storage:All kit components should be stored at -20°C.
> Storage Condition:-20°C

  • Full length double stranded blunt end cDNA synthesis for cloning
  • Double stranded cDNA library construction

Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.

Specifications

Specifications

Full-length double-stranded blunt-end cDNA synthesis for cloning
10 Reactions
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit