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Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)

Catalog No. FEREP0751
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Quantity:
10,000 Units
2,000 Units
4 x 10,000 Units

Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.

Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT. The engineered enzyme features dramatically improved thermostability, 50X higher processivity, robustness, and increased synthesis rate compared to wild type M-MuLV RT.

The eliminated RNase H activity enables the enzyme to produce very long RNA transcripts up to 20 kb. Due to its high thermostability, the enzyme maintains full activity during the entire reverse transcription reaction and generates high yields of cDNA. The reaction temperature can be increased to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. The extremely high processivity of Maxima H Minus enzyme results in increased resistance to common reaction inhibitors, such as guanidine, formamide, and ethanol. 

Features of Maxima H Minus Reverse Transcriptase include:
• Thermostable—90% active after incubation at 50°C for 60 minutes in a reaction mixture
• Active up to 65°C
• High yields of full-length cDNA up to 20 kb
• High sensitivity—reproducible cDNA synthesis from a wide range of starting total RNA amounts (1 pg to 5 μg)
• Efficient—complete cDNA synthesis in 15 to 30 minutes
• Increased resistance to common reaction inhibitors
• Incorporates modified nucleotides

Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• Analysis of RNA by primer extension

Related products
• Maxima H Minus Reverse Transcriptase (Cat. No. EP0752)
• Maxima H Minus First Strand cDNA Synthesis Kit (Cat. No. K1652)
• Maxima H Minus First Strand cDNA Synthesis Kit, with dsDNase (Cat. No. K1681)
• Maxima H Minus cDNA Synthesis Master Mix (Cat. No. M1661)
• Maxima H Minus cDNA Synthesis Master Mix, with dsDNase (Cat. No. M1681)

Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.

TRUSTED_SUSTAINABILITY

Specifications

Concentration 200 U/μL
Content And Storage

• Maxima H Minus Reverse Transcriptase, 1 x 2000 units (200 U/μL)
• 5X RT Buffer

Store at –20°C.

Format Stand-alone Enzyme
GC-Rich PCR Performance High
Reaction Speed 15 to 30 min.
Technique Reverse Transcription
Optimal Reaction Temperature 50°C to 55°C
Quantity 2,000 Units
Reverse Transcriptase Maxima H Minus
Ribonuclease H Activity Reduced
Shipping Condition Dry Ice
Final Product Type First-Strand cDNA
Reaction Format Separate components
Reagent Type Reverse Transcription
Size (Final Product) Up to 20 kb
Starting Material RNA
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WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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