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Cytiva Thermo Sequenase DNA Polymerase

Novel thermostable DNA polymerase that uses dideoxynucleotide triphosphates (ddNTPs) as readily as deoxynucleotide (dNTP) substrates

$1038.00 - $6735.00

Specifications

For Use With (Application) DNA Sequencing, and SNP Genotyping Applications
Storage Requirements -20°C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol
Includes Thermo Sequenase with Thermoplasma Acidophilum Inorganic Pyrophosphatase (TAP), Dilution Buffer, and Reaction Buffer
Source E. coli clones that overproduce the protein
No. of Reactions 32 units/µL
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
09-928-126
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Cytiva
E79000Y
1,000 Units
Each for $1,038.00
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09-928-127
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Cytiva
E79000Z
10,000 Units
Each for $6,735.00
Add to cart
 
Description

Description

  • Uniform and easy-to-read band patterns
  • Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences
  • Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing
  • ddNTPs are chain-elongating inhibitors of DNA polymerase, which are used in the Sanger method for DNA sequencing
  • Each nucleotide base of this particular type has a probability of being bonded not to a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation
  • An excellent choice for cycle sequencing where template quantity limitations preclude the use of T7 Sequenase DNA polymerase
  • Can also be used in many SNP genotyping, genotyping by sequencing, or genome sequencing applications where primer extension is employed
Specifications

Specifications

DNA Sequencing, and SNP Genotyping Applications
Thermo Sequenase with Thermoplasma Acidophilum Inorganic Pyrophosphatase (TAP), Dilution Buffer, and Reaction Buffer
32 units/µL
-20°C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol
E. coli clones that overproduce the protein
SDS
Product Certifications
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