Thymidylate Synthase Antibody - BSA Free, Novus Biologicals™

Sheep Polyclonal Antibody

Supplier:  Novus Biologicals NB600642100UG

Catalog No. NB600642100

Description

Thymidylate Synthase Polyclonal specifically detects Thymidylate Synthase in Human samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Thymidylate Synthase
• Classification: Polyclonal
• Dilution: Western Blot 1:500-1:2000, ELISA 1:10000-1:50000
• Gene Alias: EC 2.1.1.45, HsT422, MGC88736, thymidylate synthase, thymidylate synthetase, TMSTsase, TSase, TSHST422
• Host Species: Sheep
• Purification Method: IgG purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. The antibody recognizes the 36,000 MW hTS. In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required for some non-human cell lines. Anti-Thymidylate Synthase can detect TS by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining, however, some colorectal cancer specimens show very strong staining. Reactivity in other immunoassays is unknown.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG

• Applications: Western Blot, ELISA
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Symbols: TYMS
• Immunogen: Recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.
• Quantity: 100 μg
• Research Discipline: Cancer, Cell Cycle and Replication, DNA Repair, DNA replication Transcription Translation and Splicing
• Gene ID (Entrez): 7298
• Target Species: Human
• Form: Purified

For Research Use Only