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Thymidylate Synthase Antibody - BSA Free, Novus Biologicals™
SDP

Catalog No. NB600642100 Shop All R&D Systems Products
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NB600642100 100 μg
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Catalog No. NB600642100 Supplier Novus Biologicals Supplier No. NB600642100UG
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Description

Sheep Polyclonal Antibody

Thymidylate Synthase Polyclonal specifically detects Thymidylate Synthase in Human samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

Antigen Thymidylate Synthase
Applications Western Blot, ELISA
Classification Polyclonal
Conjugate Unconjugated
Dilution Western Blot 1:500-1:2000, ELISA 1:10000-1:50000
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
Gene Alias EC 2.1.1.45, HsT422, MGC88736, thymidylate synthase, thymidylate synthetase, TMSTsase, TSase, TSHST422
Gene Symbols TYMS
Host Species Sheep
Immunogen Recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.
Purification Method IgG purified
Quantity 100 μg
Regulatory Status RUO
Research Discipline Cancer, Cell Cycle and Replication, DNA Repair, DNA replication Transcription Translation and Splicing
Primary or Secondary Primary
Gene ID (Entrez) 7298
Test Specificity The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. The antibody recognizes the 36,000 MW hTS. In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required for some non-human cell lines. Anti-Thymidylate Synthase can detect TS by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining, however, some colorectal cancer specimens show very strong staining. Reactivity in other immunoassays is unknown.
Target Species Human
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG
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For Research Use Only

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