Novus Biologicals™ Human Tie-2 ELISA Kit (Colorimetric)

Description

ELISA kits are ideal for the quantification of target antigens in tissue extracts, serum and cell culture.

The Human Tie-2 ELISA Kit (Colorimetric) measures human Tie-2 in cell culture supernatant, serum, plasma (edta, citrate, heparin).

Specifications

• Assay Range: 0.16 - 10.0ng/mL
• Assay Sensitivity: 0.03ng/mL
• Product Type: ELISA Kit
• Sample Type: Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
• Specificity: The assay detects both natural and recombinant human TIE-2. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected, notably not with TIE-1.
• For Use With (Application): ELISA
• Gene ID (Entrez): 7010
• Gene Symbol: TEK
• Kit Contents: Aluminum pouch(es) with a Microwell Plate coated with polyclonal antibody to human TIE-2, Biotin-Conjugate anti-human TIE-2 polyclonal antibody, Streptavidin-HRP, Human TIE-2 Standard lyophilized, 20ng/mL upon reconstitution, Sample Diluent, Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA), Wash Buffer Concentrate 20x (PBS with 1% Tween 20), Substrate Solution (tetramethyl-benzidine), Stop Solution (1M Phosphoric acid), Adhesive Films
• Quantity: 96 Tests
• Research Discipline: Angiogenesis, Cancer, Protein Kinase
• Storage Requirements: Storage of components varies. See protocol for specific instructions.
• Synonym: angiopoietin-1 receptor, CD202B, CD202b antigen, EC 2.7.10, EC 2.7.10.1, hTIE2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, TEK tyrosine kinase, endothelial, TIE-2, TIE2CD202b, Tunica interna endothelial cell kinase, Tyrosine-protein kinase
• Target: Tie-2