TLR4 Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-199-2316	Rockland Immunochemicals 600401MK3	100 μg
50-199-2317	Rockland Immunochemicals 600401MK3S	25 μL

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Directed against human TLR4 protein. A BLAST analysis was used to suggest reactivity with TLR4 protein from human based on 100% homology with the immunizing sequence. The analysis also shows 100% cross-reactivity with gorilla and bonobo, and canine species.

TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.

Specifications

• Antigen: TLR4
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: O00206
• Gene ID (Entrez): 7099
• Primary or Secondary: Primary
• Product Type: Antibody

• Concentration: 1 mg/mL
• Classification: Polyclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Alias: ARMD10; BOS_9391; CD284; EGK_07381; homolog of Drosophila toll; hToll; lipopolysaccharide response; Lps; Ly87; Ran/M1; Rasl2-8; Tlr4; TLR-4; TOLL; toll like receptor 4; Toll4; Toll-like receptor 4; Toll-like receptor 4-like protein; Toll-like receptor4 protein
• Gene Symbols: TLR4
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Gene: TLR4