TNF-alpha Antibody (J2D10), Alexa Fluor™ 594, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP247677AF594

Catalog No. NP247677A94

Description

TNF-alpha Monoclonal specifically detects TNF-alpha in Human, Rat, Mouse (Negative) samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence.

Specifications

• Antigen: TNF-alpha
• Classification: Monoclonal
• Conjugate: Alexa Fluor 594
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: TNF
• Immunogen: Recombinant human TNF-alpha (Uniprot: P01375)
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Test Specificity: This antibody neutralizes Hu rTNFa mediated cytotoxicity of L929 cells and inhibits tumor growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF-alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF-alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF-alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF-alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF-alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF-alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF-alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
• Content And Storage: Store at 4°C in the dark.

• Applications: Flow Cytometry, Immunofluorescence
• Clone: J2D10
• Dilution: Flow Cytometry, Immunofluorescence
• Gene Alias: APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Research Discipline: Adaptive Immunity, Asthma, Autoimmune Diseases, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
• Gene ID (Entrez): 7124.0
• Target Species: Human, Rat, Mouse
• Isotype: IgG1 κ