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TNF-alpha Antibody (J2D10), Alexa Fluor™ 594, Novus Biologicals™
SDP

Catalog No. NP247677A94 Shop All R&D Systems Products
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Catalog No. NP247677A94 Supplier Novus Biologicals Supplier No. NBP247677AF594
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Description

Mouse Monoclonal Antibody

TNF-alpha Monoclonal specifically detects TNF-alpha in Human, Rat, Mouse (Negative) samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence.

Specifications

Antigen TNF-alpha
Applications Flow Cytometry, Immunofluorescence
Classification Monoclonal
Clone J2D10
Conjugate Alexa Fluor 594
Dilution Flow Cytometry, Immunofluorescence
Formulation 50 mM sodium borate with 0.05% sodium azide
Gene Alias APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Gene Symbols TNF
Host Species Mouse
Immunogen Recombinant human TNF-alpha (Uniprot: P01375)
Purification Method Protein A or G purified
Quantity 0.1 mL
Research Discipline Adaptive Immunity, Asthma, Autoimmune Diseases, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
Primary or Secondary Primary
Gene ID (Entrez) 7124.0
Test Specificity This antibody neutralizes Hu rTNFa mediated cytotoxicity of L929 cells and inhibits tumor growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF-alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF-alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF-alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF-alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF-alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF-alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF-alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
Target Species Human, Rat, Mouse
Content And Storage Store at 4°C in the dark.
Isotype IgG1 κ
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