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Human TNF-alpha Antibody, R&D Systems™
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Catalog No. MAB2101100
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MAB2101100 100 μg
MAB2101SP 25 μg
MAB2101500 500 μg
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Mouse Monoclonal Antibody has been used in 6 publications

TNF-alpha Monoclonal specifically detects TNF-alpha in Human samples. It is validated for Flow Cytometry, Neutralization, CyTOF-ready.

Specifications

Antigen TNF-alpha
Applications Flow Cytometry, Neutralization, CyTOF
Classification Monoclonal
Clone 6401
Concentration LYOPH
Conjugate Unconjugated
Dilution Flow Cytometry 0.25 ug/10^6 cells, Neutralization 0.015-0.06 ug/mL, CyTOF-ready
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Accession No. P01375
Gene Alias APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Gene Symbols TNF
Host Species Mouse
Immunogen E. coli-derived recombinant human TNF-alpha Gly57-Leu233 (predicted) Accession # P01375
Purification Method Protein A or G purified from ascites
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 7124
Test Specificity Detects human TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25-50% cross-reactivity with recombinant porcine TNF-alpha and recombinant rhesus macaque TNF-alpha is observed but no cross-reactivity with recombinant cotton rat TNF-alpha , recombinant rat TNF-alpha , recombinant human (rh) LT alpha 1/beta 2, rhLT alpha 2/beta 1, rhAPRIL, rhBAFF, rhEDA-A2, recombinant mouse EDA, rhFas Ligand, rhLIGHT, rhOX40 Ligand, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Target Species Human
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Form Purified
Isotype IgG1
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