TRAF-2 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 3 publications

Supplier:  Novus Biologicals NB10056173

Catalog No. NB10056173

Description

TRAF-2 Polyclonal specifically detects TRAF-2 in Human samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin.

Specifications

• Antigen: TRAF-2
• Classification: Polyclonal
• Dilution: Western Blot 1:1000-1:2000, Flow Cytometry, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:1000-1:5000
• Gene Alias: E3 ubiquitin-protein ligase TRAF2, EC 6.3.2.-, MGC:45012, TNF receptor-associated factor 2, TRAP3TRAP, tumor necrosis factor type 2 receptor associated protein 3, Tumor necrosis factor type 2 receptor-associated protein 3
• Host Species: Rabbit
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: The gene encoding TRAF2 produces, by alternative splicing, 15 different transcripts, all with introns, putatively encoding 15 different protein isoforms. These isoforms are of varying amino acid lengths. Therefore the molecular weight observed by western blot may vary, depending on the isoform expressed. Users are encouraged to use the peptide immunogen sequence information provided in the antigen section to gain additional information about the potential TRAF2 isoforms detected by the antibody.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Conjugate: Unconjugated
• Formulation: Whole antisera with 0.02% Sodium Azide
• Gene Symbols: TRAF2
• Immunogen: A synthetic peptide corresponding to amino acids 6-31 (VTPPGSLELLQPGFSKTLLGTKLEAK) of human TRAF2 was used as immunogen, GenBank no. NP_066961.2. This peptide sequence is 92% conserved in mouse and rat TRAF2. The peptide immunogen sequence is 100% conserved in multiple human TRAF2 GenBank accession numbers. These TRAF2 accession numbers are of varying amino acid (aa) lengths and include: 1) NP_066961.2 = 501 aa. 2) CAI12705.1 =121 aa. 3) CAI12704.1 = 422 aa. 4) CAI15104.1 = 193 aa. 5) BAD96681.1 = 205 aa.
• Quantity: 0.05 mL
• Research Discipline: Apoptosis, Cancer, Cytokine Research, Zinc Finger
• Gene ID (Entrez): 7186
• Target Species: Human
• Form: Antisera

For Research Use Only