TRAF-2 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 3 publications

Catalog Number	Mfr. No.	Quantity
NB10056173T	Novus Biologicals NB10056173SS	0.025 mL
NB10056173	Novus Biologicals NB10056173	0.05 mL

Description

TRAF-2 Polyclonal specifically detects TRAF-2 in Human samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin.

Specifications

• Antigen: TRAF-2
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Conjugate: Unconjugated
• Host Species: Rabbit
• Research Discipline: Apoptosis, Cancer, Cytokine Research, Zinc Finger
• Formulation: Whole antisera with 0.02% Sodium Azide
• Gene ID (Entrez): 7186
• Immunogen: A synthetic peptide corresponding to amino acids 6-31 (VTPPGSLELLQPGFSKTLLGTKLEAK) of human TRAF2 was used as immunogen, GenBank no. NP_066961.2. This peptide sequence is 92% conserved in mouse and rat TRAF2. The peptide immunogen sequence is 100% conserved in multiple human TRAF2 GenBank accession numbers. These TRAF2 accession numbers are of varying amino acid (aa) lengths and include: 1) NP_066961.2 = 501 aa. 2) CAI12705.1 =121 aa. 3) CAI12704.1 = 422 aa. 4) CAI15104.1 = 193 aa. 5) BAD96681.1 = 205 aa.
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Dilution: Western Blot 1:1000-1:2000, Flow Cytometry, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:1000-1:5000
• Classification: Polyclonal
• Form: Antisera
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: E3 ubiquitin-protein ligase TRAF2, EC 6.3.2.-, MGC:45012, TNF receptor-associated factor 2, TRAP3TRAP, tumor necrosis factor type 2 receptor associated protein 3, Tumor necrosis factor type 2 receptor-associated protein 3
• Gene Symbols: TRAF2
• Isotype: IgG
• Purification Method: Unpurified
• Test Specificity: The gene encoding TRAF2 produces, by alternative splicing, 15 different transcripts, all with introns, putatively encoding 15 different protein isoforms. These isoforms are of varying amino acid lengths. Therefore the molecular weight observed by western blot may vary, depending on the isoform expressed. Users are encouraged to use the peptide immunogen sequence information provided in the antigen section to gain additional information about the potential TRAF2 isoforms detected by the antibody.

For Research Use Only