Novus Biologicals™ TRAIL/TNFSF10 Antibody Pair [HRP]

Description

Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Human TRAIL/TNFSF10.

Specifications

• Assay Range: 31.25 pg/mL
• Assay Sensitivity: 31.25-2000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Human TRAIL/TNFSF10.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: Apo-2 ligand, APO2L, Apo-2Ltumor necrosis factor (ligand) family, member 10, APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta, CD253, CD253 antigen, Protein TRAIL, TL2, TNF-related apoptosis-inducing ligand, TRAILTNF-related apoptosis inducing ligand TRAIL, tumor necrosis factor (ligand) superfamily, member 10, tumor necrosis factor ligand superfamily member 10
• Gene ID (Entrez): 8743
• Gene Symbol: TNFSF10
• Host Species: Rabbit/Mouse
• Kit Contents:
  Standard: Each vial contains 25(KW10AP902-1) ng of recombinant Human TRAIL/TNFSF10.
  Capture Antibody: 0.4 mg/mL of mouse anti-Human TRAIL/TNFSF10 monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.1 mg/mL of rabbit anti-Human TRAIL/TNFSF10 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Apoptosis, Cancer, Cell Biology, Death Receptor Signaling Pathway, Signal Transduction, Tumor Suppressors
• Storage Requirements: Storage is content dependent.
• Target: TRAIL/TNFSF10
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.