TRF-2 Antibody (4A794.15) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NB10056506T	Novus Biologicals NB10056506SS	0.025 mg
NB10056506	Novus Biologicals NB10056506	0.1 mg

Description

TRF-2 Monoclonal specifically detects TRF-2 in Human, Mouse, Rat, Deer, Marsupial samples. It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Proximity Ligation Assay, CyTOF-ready.

Specifications

• Antigen: TRF-2
• Concentration: 1 mg/mL
• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunohistochemistry
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cell Cycle and Replication, Cellular Markers, Chromatin Research, Transcription Factors and Regulators
• Gene Accession No.: Q15554
• Gene ID (Entrez): 7014
• Immunogen: This TRF-2 Antibody (4A794.15) was developed against Baculovirus expressed whole length TRF2 protein, used for immunizing mice and splenocytes used to generate the hybridoma clone (NP_005643).
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 59.6 kDa

• Clone: 4A794.15
• Dilution: Western Blot 2-4 ug/ml, Simple Western 1:50, Flow Cytometry 0.1 ug/10^6 cells, ELISA 2 ug/ml, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 2 ug/10^6 cells, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen 5 ug/ml, Immunoblotting reported in scientific literature (PMID 23708666), Proximity Ligation Assay reported in scientific literature (PMID 27366950), Chromatin Immunoprecipitation (ChIP) 1:10-1:500, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Deer, Marsupial
• Gene Alias: 4A794, 4A794 trf2, 4A794.15, 4A794.15 trf2, anti-trf2 4A794, anti-trf2 4A794.15, clone 4A794, clone 4A794.15, Telomeric DNA-binding protein, telomeric repeat binding factor 2, Telomeric repeat binding protein 2, telomeric repeat-binding factor 2, TERF 2, TERF2, TRBF 2, TRBF2, TRF 2, TRF2, TTAGGG repeat binding factor 2, TTAGGG repeat-binding factor 2
• Gene Symbols: TERF2
• Isotype: IgG1 κ
• Purification Method: Protein G purified
• Test Specificity: The TRF2 antibody recognizes full-length TRF2 as well as TRF2 forms lacking both the N-terminal basic domain (B) and the telobox Myb-like C-terminal DNA-binding domain (M). TRF2 forms missing the B and M domains are often referred to as mutant TRF2. Although the exact epitope recognized by the TRF2 antibody has not been mapped, the scientific literature indicates it is in the D or L domain, but not in the B or M domain.

For Research Use Only