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Applied Biosystems™ TrueMark™ Respiratory I Plus Combo Kit

Catalog No. A56284C
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The Applied Biosystems TrueMark Respiratory I Plus Combo Kit achieves molecular detection through real-time PCR technology, offering an end-to-end workflow for many common infectious disease areas by utilizing the power of QuantStudio real-time PCR systems.

The Applied Biosystems TrueMark Respiratory I Plus Combo Kit achieves molecular detection through real-time PCR technology, offering an end-to-end workflow for many common infectious disease areas by utilizing the power of QuantStudio real-time PCR systems. The combination of a TrueMark panel (included) and a QuantStudio system helps enable rapid, accurate detection and categorization of microorganisms that cause respiratory diseases.

The TrueMark Respiratory I Plus panel is composed of convenient, duplexed TaqMan assays that detect adenovirus, enterovirus, influenza A (Pan), influenza B, respiratory syncytial virus A/B, rhinovirus, human metapneumovirus, SARS-CoV-2, and an internal control in the same reaction, utilizing real-time PCR techniques.

Features of the TrueMark Respiratory I Plus panel include:
Ease-of-use—convenient, duplexed reactions include assay and positive control, pre-spotted and dried down on TaqMan plates, designed for easy setup and increased accuracy; just add sample and TrueMark Infectious Disease Panels 1-Step Multiplex Master Mix (No ROX) (included)
Endogenous controls—duplex reactions offer RNase P human internal control in every well for added confidence
Turn-around time—choose from a selection of predefined panels with a short turn-around time
Amplification control—TrueMark Amplification Control I covers all targets in RTI, UTI, STI, and vaginal panels
Maximize the workflow—expand from SARS-CoV-2 testing to TrueMark infectious disease research panels utilizing the same workflow

The TrueMark Respiratory I Plus Combo Kit includes the following components:
TrueMark Respiratory I Plus Panel—collection of duplex assays that contain primer/probe sets specific to adenovirus, enterovirus, influenza A (Pan), influenza B, respiratory syncytial virus A/B, rhinovirus, human metapneumovirus, SARS-CoV-2, and RNase P genomic regions
TrueMark Infectious Disease Panels 1-Step Multiplex Master Mix (No ROX)—ready-to-use PCR mix, including reverse transcriptase, polymerase, dNTPs, salt, and buffer

The TrueMark Respiratory I Plus Combo Kit is used with nasopharyngeal swab or nasopharyngeal aspirate samples on our QuantStudio real-time PCR systems.

Product use
The TrueMark Respiratory I Plus Panel is a collection of duplex assays that detect microbes and an internal control in the same reaction for qualitative detection and identification of adenovirus, enterovirus, influenza A (Pan), influenza B, respiratory syncytial virus A/B (RSV A/B), rhinovirus, human metapneumovirus (HM), SARS-CoV-2 in nasopharyngeal (NP) or nasopharyngeal aspirate swabs obtained from individuals exhibiting signs and symptoms of a respiratory tract infection.

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Specifications

Content And Storage 5 plates; 3 x 1 mL
Store panels at 15–30°C.
Store master mix at 5 to -30°C.

Detection Method Primer-probe
Label or Dye 2 Color
Quantity 5 Plates kit
Target Adenovirus, enterovirus, influenza A (Pan), influenza B, respiratory syncytial virus A/B, rhinovirus, human metapneumovirus, and SARS-CoV-2
For Use With (Application) Pathogen Detection
Compatibility QuantStudio™ 5 Real‐Time PCR System, 96-well, 0.2-mL block; QuantStudio™ 5 Dx Real‐Time PCR System, 96-well, 0.2-mL block; QuantStudio™ 7 Pro Real-Time PCR System, 96-well, 0.2-mL block
No. of Reactions Up to 12 Samples/Plate
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