Tyrosinase Antibody (T311 + OCA1/812), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2446860.1MG

Catalog No. NBP24468601

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Tyrosinase Monoclonal specifically detects Tyrosinase in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: Tyrosinase
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
• Gene Alias: CMM8, EC 1.14.18.1, LB24-AB, Monophenol monooxygenase, OCA1A, OCAIA, SHEP3, SK29-AB, Tumor rejection antigen AB, tyrosinase, tyrosinase (oculocutaneous albinism IA)
• Host Species: Mouse
• Molecular Weight of Antigen: 75 kDa
• Quantity: 0.1 mg
• Research Discipline: Lipid and Metabolism
• Gene ID (Entrez): 7299
• Target Species: Human
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: T311 + OCA1/812
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Symbols: TYR
• Immunogen: Recombinant tyrosinase protein (T311); Recombinant human TYR protein (OCA1/812)
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.
• Content And Storage: Store at 4C.
• Isotype: IgG

For Research Use Only