Tyrosinase Antibody (T311 + OCA1/812), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24468600	Novus Biologicals NBP2446860.02MG	0.02 mg
NBP24468601	Novus Biologicals NBP2446860.1MG	0.1 mg
NBP24468602	Novus Biologicals NBP2446860.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Tyrosinase Monoclonal specifically detects Tyrosinase in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: Tyrosinase
• Concentration: 0.2mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Lipid and Metabolism
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene ID (Entrez): 7299
• Immunogen: Recombinant tyrosinase protein (T311); Recombinant human TYR protein (OCA1/812)
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 75 kDa

• Clone: T311 + OCA1/812
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: CMM8, EC 1.14.18.1, LB24-AB, Monophenol monooxygenase, OCA1A, OCAIA, SHEP3, SK29-AB, Tumor rejection antigen AB, tyrosinase, tyrosinase (oculocutaneous albinism IA)
• Gene Symbols: TYR
• Isotype: IgG
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.

For Research Use Only