Ubiquitin Antibody (Ubi-1), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 32 publications

Supplier:  Novus Biologicals NB300130

Catalog No. NB300130

Description

Ubiquitin Monoclonal specifically detects Ubiquitin in Human, Mouse, Rat, Porcine, Bovine, C. elegans, Chicken, Drosophila, Equine, Plant, Monkey, Zebrafish samples. It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting.

Specifications

• Antigen: Ubiquitin
• Classification: Monoclonal
• Concentration: 1 mg/ml
• Dilution: Western Blot 1:1000-1:2000, Immunohistochemistry 1:2000, Immunocytochemistry/Immunofluorescence 1:2000, Immunohistochemistry-Paraffin 1:2000, Immunohistochemistry-Frozen 1:2000, Immunoblotting
• Gene Accession No.: P0CG48, P0CG47
• Gene Symbols: RPS27A
• Immunogen: Purified bovine erythrocyte ubiquitin cross-linked with glutaraldehyde to KLH
• Purification Method: Affinity purified
• Regulatory Status: RUO
• Gene ID (Entrez): 6233
• Target Species: Human, Mouse, Rat, Pig, Bovine, C. elegans, Chicken, Drosophila, Equine, Plant, Monkey, Zebrafish
• Form: Ascites

• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot
• Clone: Ubi-1
• Conjugate: Unconjugated
• Formulation: 50% PBS, 50% glycerol with 5mM Sodium Azide
• Gene Alias: RPS27A, UBA52, UBB ubiquitin B, UBC
• Host Species: Mouse
• Molecular Weight of Antigen: 8.5 kDa
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Test Specificity: Recognizes polyubiquitin chains much more strongly than monoubiquitinated molecules or free ubiquitin. Specifically recognizes ubiquitinated cytoplasmic inclusion bodies.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1

For Research Use Only