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Vimentin Antibody (SPM576), DyLight 594, Novus Biologicals™
SDP

Catalog No. NB006865 Shop All R&D Systems Products
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NB006865 0.1 mL
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Catalog No. NB006865 Supplier Novus Biologicals Supplier No. NBP234809DL594
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Description

Mouse Monoclonal Antibody

Vimentin Monoclonal specifically detects Vimentin in Human, Porcine, Bovine, Canine, Chicken, Feline, Goat, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

Antigen Vimentin
Applications Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone SPM576
Conjugate DyLight 594
Dilution Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
Formulation 50mM Sodium Borate with 0.05% Sodium Azide
Gene Alias FLJ36605, vimentin
Gene Symbols VIM
Host Species Mouse
Immunogen Human vimentin recombinant protein (Uniprot: P08670)
Purification Method Protein A or G purified
Quantity 0.1 mL
Regulatory Status RUO
Research Discipline Biologically Active Proteins, Cancer, Cell Biology, Cellular Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuroscience, Phospho Specific, Signal Transduction, Stem Cell Markers, Stem Cells
Primary or Secondary Primary
Test Specificity This monoclonal antibody reacts with a 58kDa protein identified as Vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of Vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-Vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-Vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain Vimentin, e.g. hepatoma and seminoma. Anti-Vimentin is also useful as a tissue process control reagent.
Target Species Human, Pig, Bovine, Canine, Chicken, Feline, Goat, Mouse (Negative), Rat (Negative)
Content And Storage Store at 4°C in the dark.
Isotype IgG1 κ
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