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Vimentin Antibody (VM452), Alexa Fluor™ 750, Novus Biologicals™
SDP

Catalog No. NP233060A70 Shop All R&D Systems Products
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NP233060A70 0.1 mL
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Catalog No. NP233060A70 Supplier Novus Biologicals Supplier No. NBP233060AF750
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Description

Mouse Monoclonal Antibody

Vimentin Monoclonal specifically detects Vimentin in Human, Porcine, Bovine, Canine, Chicken, Feline, Goat, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry-Paraffin, Knockout Validated.

Specifications

Antigen Vimentin
Applications Western Blot, ELISA, Immunoassay
Classification Monoclonal
Clone VM452
Conjugate Alexa Fluor 750
Dilution Western Blot, ELISA, Knockout Validated
Formulation 50 mM sodium borate with 0.05% sodium azide
Gene Alias FLJ36605, vimentin
Gene Symbols VIM
Host Species Mouse
Immunogen Recombinant full-length human vimentin protein (Uniprot: P08670)
Purification Method Protein A or G purified
Quantity 0.1 mL
Primary or Secondary Primary
Gene ID (Entrez) 7431
Test Specificity This monoclonal antibody reacts with a 58kDa protein identified as Vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of Vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-Vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-Vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain Vimentin, e.g. hepatoma and seminoma. Anti-Vimentin is also useful as a tissue process control reagent.
Target Species Human, Pig, Bovine, Canine, Chicken, Feline, Goat, Mouse, Rat
Content And Storage Store at 4°C in the dark.
Isotype IgG1 κ
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