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Vimentin Antibody (VM452) - Azide and BSA Free, Novus Biologicals™
SDP

Catalog No. NBP233060 Shop All R&D Systems Products
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NBP233060 0.1 mg
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Catalog No. NBP233060 Supplier Novus Biologicals Supplier No. NBP233060
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Description

Mouse Monoclonal Antibody

Vimentin Monoclonal specifically detects Vimentin in Human, Porcine, Bovine, Canine, Chicken, Feline, Goat, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry-Paraffin, Protein Array, Knockout Validated.

Specifications

Antigen Vimentin
Applications Western Blot, Flow Cytometry, ELISA, Immunohistochemistry (Paraffin), Peptide Array, Immunoassay
Classification Monoclonal
Clone VM452
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.5 - 2.0 ug/ml, Flow Cytometry, ELISA 1-5ug/ml for coating, Immunohistochemistry-Paraffin, Protein Array, Knockout Validated
Gene Alias FLJ36605, vimentin
Gene Symbols VIM
Host Species Mouse
Immunogen Recombinant full-length human vimentin protein (Uniprot: P08670)
Purification Method Protein A or G purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Phospho Specific, Signal Transduction, Stem Cell Markers, Stem Cells
Primary or Secondary Primary
Gene ID (Entrez) 7431
Test Specificity This monoclonal antibody reacts with a 58kDa protein identified as Vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of Vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-Vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-Vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain Vimentin, e.g. hepatoma and seminoma. Anti-Vimentin is also useful as a tissue process control reagent.
Target Species Human, Pig, Bovine, Canine, Chicken, Feline, Goat, Mouse (Negative), Rat (Negative)
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1 κ
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