Vimentin Mouse, Clone: SPM576, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2329100.2MG

Catalog No. NBP232910B

Description

Vimentin Monoclonal antibody specifically detects Antigen in Bovine, Canine, Chicken, Feline, Goat, Human, Mouse (Negative), Porcine, Rat (Negative) samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting.

Specifications

• Antigen: Vimentin
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: FLJ36605, vimentin
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
• Content And Storage: Store at 4C.
• Isotype: IgG1

• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Clone: SPM576
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
• Gene Accession No.: P08670
• Gene Symbols: VIM
• Immunogen: Human vimentin recombinant protein
• Quantity: 0.2 mg
• Research Discipline: Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Phospho Specific, Signal Transduction, Stem Cell Markers, Stem Cells
• Gene ID (Entrez): 7431
• Target Species: Human, Pig, Bovine, Canine, Chicken, Feline, Goat, Mouse (Negative), Rat (Negative)
• Form: Purified