WT1 Antibody (WT1/857), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2446060.1MG

Catalog No. NBP24460601

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

WT1 Monoclonal specifically detects WT1 in Human, Rat samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: WT1
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Accession No.: P19544
• Gene Symbols: WT1
• Immunogen: Recombinant human WT1 protein
• Quantity: 0.1 mg
• Research Discipline: Angiogenesis, Cancer, Cell Cycle and Replication, Cellular Markers, Chromatin Research, Tumor Suppressors
• Gene ID (Entrez): 7490
• Target Species: Human, Mouse, Rat
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: WT1/857
• Conjugate: Unconjugated
• Formulation: 1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: AWT1, NPHS4GUD, WAGR, Wilms tumor 1, Wilms tumor protein, WIT-2, WT33
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

For Research Use Only