ZAP70 Antibody (ZAP70/528), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2445040.02MG

Catalog No. NBP24450400

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

ZAP70 Monoclonal specifically detects ZAP70 in Human samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: ZAP70
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Flow Cytometry 0.5-1ug/million cells in 0.1ml, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunofluorescence 0.5-1ug/ml
• Gene Alias: 70 kDa zeta-associated protein, EC 2.7.10, EC 2.7.10.2, SRKFLJ17679, STDFLJ17670, Syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, TZK, ZAP-70, zeta-chain (TCR) associated protein kinase (70 kD), zeta-chain (TCR) associated protein kinase 70kDa, zeta-chain associated protein kinase, 70kD
• Host Species: Mouse
• Molecular Weight of Antigen: 70 kDa
• Quantity: 0.02 mg
• Research Discipline: Adaptive Immunity, Cell Biology, Immunology, Protein Kinase, Signal Transduction, Tyrosine Kinases
• Gene ID (Entrez): 7535
• Target Species: Human
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: ZAP70/528
• Conjugate: Unconjugated
• Formulation: 1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Symbols: ZAP70
• Immunogen: Recombinant full-length human ZAP70 protein
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells.Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well.Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
• Content And Storage: Store at 4C.
• Isotype: IgG2a κ

For Research Use Only