ZO-1 Monoclonal Antibody (R26.4C), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  LIFE TECHNOLOGIES 14977637

Catalog No. 14-977-637

Description

The MAb R26.4C recognizes the tight junction protein 1, ZO-1 (zona occludens) of rat, mouse, canine, and porcine. ZO-1 is a member of the MAGUK family of tight junction associated scaffolding proteins, each containing a PDZ, SH3 and guanylate kinase-like (GUK) domain. ZO-1 is an integral member of the tight junction complex which forms a barrier to paracellular movement of substances, separating apical and basolateral fluids in relation to the epithelial cell layer. ZO-1 forms complexes with family members ZO-2, ZO-3, as well as occludin, cingulin, claudin-1-8, actin, alpha-catenin, the Ras-effector molecule AF-6, ZONAB, Apg-2, and the junctional adhesion molecules (JAM)-A-C. ZO-1 is thought to anchor the actin cytoskeleton to the tight junction. ZO-1 and ZO-2 have been shown to regulate cell cycle and proliferation with recent evidence highlighting the down-regulation of ZO-1 and ZO-2 with cancer progression.

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

Specifications

• Antigen: ZO-1
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: O97758, P39447
• Gene Symbols: TJP1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 21872, 292994, 403752
• Content And Storage: 4° C
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot
• Clone: R26.4C
• Conjugate: Unconjugated
• Gene: TJP1
• Gene Alias: DKFZp686M05161; I79_005485; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); tight junction protein ZO-1; Tjp1; zo1; ZO-1; ZO-1 MDCK; zona occludens 1; zona occludens protein 1; zonula occludens 1; zonula occludens 1 protein; zonula occludens protein 1
• Host Species: Rat
• Quantity: 2 mg
• Primary or Secondary: Primary
• Target Species: Canine, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a κ