Bioassay Systems
BioAssay Systems develops and markets innovative and high-throughput assay solutions to satisfy the ever increasing demands of the life sciences industry.
Bioassay Systems QuantiQuik™ Urea (BUN) Quick Test Strips. Direct determination of urea concentrations in food and beverage samples as well as biological samples.
For rapid, direct determination of urea concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 µL sample. Semi-quantitative measurement between 0-1500 mg/L (undiluted) urea. Convenient. No expensive plate or cuvette readers needed. Sample trea™ent and assay can be performed in under 15 minutes. Method: Visual. Samples: Serum, plasma, urine, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.
Bioassay Systems QuantiChrom™ Arginase Assay Kit. For quantitative determination of arginase activity and screen for its inhibitors.
For quantitative determination of arginase activity and screen for its inhibitors. Key Features: Sensitive and accurate. Detection limit: 0.3 U/L for 2 hr arginase reaction in 96-well assay format. Simple and high-throughput. The procedure involves incubation of the provided substrate with the sample in a microplate followed by the addition of the coloring reagent. Can be readily automated as a high-throughput assay for thousands of samples per day. Method: OD430nm. Samples: Enzyme preparations, serum, plasma, tissue culture etc. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 tests. Detection limit: 1 U/L (0.3 U/L for 2 hr reaction).
Bioassay Systems QuantiChrom™ Protein Creatinine Ratio Assay Kit. For quantitative determination of urinary protein and creatinine concentrations (protein/creatinine ratio, UPCR).
For quantitative determination of urinary protein and creatinine concentrations (protein/creatinine ratio, UPCR). Key Features: Sensitive and accurate. Use 20 µL samples. Linear detection range in 96-well plate: 1 - 20 mg/dL Protein and 1 - 150 mg/dL Creatinine. Fast and convenient. No sample pre-trea™ent is needed. Simple 10- minute "add-incubate-read" procedure. High-throughput adaptable. The procedure can be readily automated for processing thousands of samples per day. Method: OD600 and OD530nm. Samples: Urine samples. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 1 mg/dL Protein and 1 mg/dL Creatinine.
Bioassay Systems EnzyFluo™ Bile Acid Assay Kit. For quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism.
For quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. Linear detection range of 1 - 150 µM bile acids. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: Serum, plasma, urine and other biological samples. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 1 µM.
Bioassay Systems QuantiChrom™ Whole Blood Hemoglobin Kit. For quantitative determination of hemoglobin (Hb) in whole blood.
For quantitative determination of hemoglobin (Hb) in whole blood. Key Features: Safety. Non-hazardous. Reagents do not contain any toxic components, e.g. hexacyanoferrate(III) and potassium cyanide used in Drabkin's reagent. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Versatility. Assays can be executed in 96-well plate or cuvette. High sensitivity. As low as 5 µL sample is used. Ideal when samples are limited such as mice blood samples. Method: OD570nm. Samples: Whole blood samples. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.2 g/dL.
Bioassay Systems EnzyChrom™ D-Lactate Assay Kit. For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.
For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565 nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.05 mM.
Bioassay Systems EnzyChrom™ Glutamine Assay Kit. For quantitative determination of glutamine and evaluation of drug effects on glutamine metabolism.
For quantitative determination of glutamine and evaluation of drug effects on glutamine metabolism. Key Features: Sensitive and accurate. Use 20 µL sample. Linear detection range 0.023 - 2 mM glutamine in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, incubation for 40 min at room temperature, adding a stop reagent and reading the optical density. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, urine, cell, tissue etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 23 µM.
Bioassay Systems EnzyChrom™ Pyruvate Assay Kit. For quantitative determination of pyruvate and evaluation of drug effects on its metabolism.
For quantitative determination of pyruvate and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 2 to 500 µM (17 µg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 µM for fluorimetric assays. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 2, 0.2 µM.
Bioassay Systems PiBlue™ Phosphate Assay Kit. For sensitive and high-throughput phosphate determination.
For sensitive and high-throughput phosphate determination. Key Features: Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. High sensitivity and wide detection range: detection of as little of 20 pmoles of phosphate and useful range between 0.4 µM and 50 µM phosphate. Fast and convenient: single reagent "mix-and-measure". Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. Robust and amenable to HTS: Z factors of 0.7 to 0.9 are observed in 96-well plates. Can be readily automated on HTS liquid handling systems Method: OD620nm (malachite green). Samples: Enzyme reactions with free phosphate releases. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 0.4 µM.
Bioassay Systems EnzyLight™ ADP/ATP Ratio Assay Kit. For quantitative bioluminescent assay for ADP:ATP ratio (apoptosis) in cells.
For quantitative bioluminescent assay for ADP:ATP ratio (apoptosis) in cells and screen for modulators. Key Features: Safe. Non-radioactive assay. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of 0.5 and above are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests.
Bioassay Systems EnzyChrom™ Sialic Acid Assay Kit. For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism.
For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.02 to 1 mM sialic acid for colorimetric assays and 2 to 100 µM for fluorimetric assays. Simple and convenient. Can detect free sialic acid by addition of a single working reagent and incubation for 60 min at room temperature or total sialic acid by pre-treating samples with a 60 min hydrolysis step. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 tests. Detection limit: OD, FL: 20, 2 µM.
Bioassay Systems EnzyChrom™ NAD/NADH Assay Kit. For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.
For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. Key Features: Sensitive and accurate. Detection limit of 0.05 µM and linearity up to 10 µM NAD+/NADH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 15 min at room temperature. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Cell or tissue extracts. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.05 µM.
Bioassay Systems CellQuanti-Blue™ Cell Viability Assay Kit (5000 tests). Homogeneous assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents.
Homogeneous assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents. Key Features: Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay). Sensitive and accurate. As low as 100 cells can be accurately quantified. Time efficient. High-throughput assay in 96-well and 384-well plates allows simultaneous processing ten of thousands of samples per day. Homogeneous and convenient. A single reagent and "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS. Z factors of 0.6 to 0.9 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: FL530/590nm. Samples: Cell culture. Species: All. Procedure: Assay takes 1-5 hrs, hands-on time 30 min. Kit size: 5000 tests. Detection limit: 100 cells.
Bioassay Systems QuantiChrom™ GTPase Assay Kit. For quantitative determination of ATPase, GTPase or any enzyme activity that liberated free phosphate and high-throughput screen for their inhibitors.
For quantitative determination of ATPase or GTPase activity and high-throughput screen for their inhibitors. Key Features: High sensitivity. Detection of 0.007 U/L ATPase or GTPase activity. Fast and convenient: single reagent, homogeneous "mix-and-measure" assay allows quantitation of enzyme activity within 30 minutes. Robust and amenable to HTS: detection at 620nm greatly reduces potential interference by colored compounds. Z factors of >0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: OD620nm. Samples: ATPase and GTPase. Species: All. Procedure: Assay takes 30 min. Kit size: 200 tests. Detection limit: 0.007 U/L.
Bioassay Systems QuantiChrom™ Formaldehyde Assay Kit. Direct determination of formaldehyde concentrations in biological, food and environmental samples
For quantitative and direct determination of formaldehyde concentrations in biological, food and environmental samples. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 1.5 µM (45 ppb) formaldehyde can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL370/470nm. Samples: Biological, food, beverage, environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 1.5 µM (45 ppb).