Histology Reagents
Electron Microscopy Sciences 10% Glutaraldehyde in Methanol 10 ML
C₅H₈O₂
Rapid-Freezing on a polished metal surface, at the temp. of liquid helium or liquid nitrogen, followed by freeze-substitution in osmium-acetone, has been shown to be a very useful technique for capturing rapid cellular events and for preserving cell ultrastructure.
Electron Microscopy Sciences Paraformaldehyde, Prills 500 GR
(Polyoxymethylene)
OH(CH₂O)n H F.W. 600 Average
Paraformaldehyde is the condensation product of methylene glycol, a substance not so far isolated in its pure form; it is a white solid with a formaldehyde content between 78 and 98% and is supplied either as a Prill or Granular. All forms contain more than 95.5% paraformaldehyde content. The Prill and Granular forms are small, free-flowing, dust-free beads, the most desired by EM users.
Electron Microscopy Sciences Styrene, Reagent 450 ML
(Vinylbenzene)
CAS #100-42-5 C₆H₅CH:CH₂ F.W. 104.14
Assay: >99.0%
A monomeric styrene used as an embedding media for ultramicrotomy. (DeLamater, E.D., et al 1971). A styrene-methacrylate resin embedding method for ultrathin sectioning ( J. Electron Micro., 10:16)
E K Industries Reagent Alcohol, 95%, 4 Gal Case
Melting point / freezing point ~-114 C, boiling range 78 C - 80 C, Flash point ~13 C
Electron Microscopy Sciences Trump's Fixative 500 ML
A combination of Sodium Cacodylate, Formalin, and Glutaraldehyde. Ready to use.
Electron Microscopy Sciences Glutaraldehyde Aqueous 50%, Biological Grade 5 1 LT
OCHCH₂CH₂CH₂CHO F.W. 100.12
Aqueous glutaraldehyde, the universal fixative for electron microscopy, is purified to EM grade from commercial grade by "all-glass vacuum-distillation". It is the highest possible quality, free from polymers and other contaminents. Each lot is assayed prior to shipping assuring consistent purity and the highest possible quality.
If stored properly, our Glutaraldehyde has a very long shelf life. EM grade aqueous glutaraldehyde, stored in volumes greater than 100 ml, with repeated defrosting and recapping, will polymerize in time, producing an absorption peak of 235nm rather than the 280nm of pure EM grade. The presence of polymerized glutaraldehyde greatly reduces its efficiency as a cross-linking agent, preventing proper fixation. For this reason, we provide 10 ml ampoules sealed under dry nitrogen and 100 ml bottles that are kept refrigerated until it is shipped.
Grade: Biological 5
Volu Sol Acid Alcohol Decolorizer, 128 oz / 3.78 L, Volu-Sol™
Decolorizer, for use in staining Acid Fast Bacteria in the Kinyoun, Modified Kinyoun, or Ziehl-Neelsen staining protocols
Electron Microscopy Sciences Histo-Clear II Non-toxic Clearing Agent 5 GL
Histo-Clear (Distilled essential oils –food grade) and Histo-Clear II (A mixtureof Aliphatic Hydrocarbon and Distilled essential oils – food grade, reduced citrus odor) are excellent non-toxic clearing agents replacing Xylene.
Electron Microscopy Sciences 8% Paraformaldehyde (formaldehyde) aqueous solution, 100mL
Paraformaldehyde - methanol free solution. A more efficient and rapid penetrant fixative used in combination with Glutaraldehyde and Acrolein fixatives. 100 ml bottles.
Electron Microscopy Sciences Formaldehyde Glutaraldehyde 3% Sodium Cacodylate Buffer, 7.4 pH 10 ML
Premixed, prebuffered fixatives, are pH adjusted for immediate laboratory use. They are freshly prepared upon receipt of order to ensure maximum shelf life. The fixatives are filled in 20 ml glass vials (10 ml/vial). This allows for the immediate fixation of your specimens without costly and time consuming transfer associated with ampoule contained fixatives. In Sodium Cacodylate.
pH: 7.4
Electron Microscopy Sciences Collodion (Parlodion), 2% in Amyl Acetate 30 ML
Highly purified nitrocellulose (parlodion strip) in glass distilled amyl acetate. Useful for forming a negative replica to very fine detail. Two types are available: Our sterile formula which is filtered down to 0.45 micron and our non-sterile formula. Non-Filter 2% Collodion. 30 ml container avoids hazardous shipping.
Sigma Aldrich Fine Chemicals Biosciences Formaldehyde solution, 50-00-0, MFCD00003274, 4x25mL
Molecular Formula: HCHO, Molecular Weight: 30.03, Beilstein Registry Number: 1209228, Concetration: 36.5-38% in H2O
![Polysciences, Inc. Glutaraldehyde; Polysciences; EM Grade; 25%; Stable; Clear colorless liquid acrid odor; Stored at 4 deg. C; Refractive index: 1.375; Density: 1.062; CAS: [111-30-8]; Size: 10 x 10mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/OS-01909-10.JPG-250.jpg)
Polysciences, Inc. Glutaraldehyde; Polysciences; EM Grade; 25%; Stable; Clear colorless liquid acrid odor; Stored at 4 deg. C; Refractive index: 1.375; Density: 1.062; CAS: [111-30-8]; Size: 10 x 10mL
Glutaraldehyde; Polysciences; EM Grade; 25%; Stable; Clear colorless liquid acrid odor; Stored at 4 deg. C; Refractive index: 1.375; Density: 1.062; CAS: [111-30-8]; Size: 10 x 10mL
Electron Microscopy Sciences Bismuth Subnitrate, Crystalline 25 GR
(Bismuth Nitrate, Basic; Bismuth Oxynitrate Hydrate)
CAS #1304-85-4 4BiNO₃(OH₂).BiO(OH) F.W. 1461.99
Bismuth staining for light and electron microscopy. M .Locke and P. Huie, Tissue & Cell, 9, 347 (1977); G.L. Brown & M. Locke, ibd. 10, 365 (1978)
Electron Microscopy Sciences ERL 4221 Cycloaliphatic Epoxide Resin 225 ML
Due to the supply shortage of ERL-4206 ERL-4221 has now been specially formulated to replace it. ERL 4206 is one of the main components of the low viscosity (Dr. Spur) embedding resin kit. ERL-4221 is a Cycloaliphatic Epoxide Resin.