This rabbit monoclonal antibody recognizes endogenous levels of total neutrophil elastase, a hematopoietic serine protease that plays a critical role in innate immunity. It is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of the human protein. The antibody is supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C; do not aliquot.
- Validated for Western blotting, immunoprecipitation, immunofluorescence (immunocytochemistry), and flow cytometry (fixed/permeabilized) applications.
- Species reactivity is limited to human.
- Recommended dilutions: 1:1,000 for Western blotting, 1:100 for immunoprecipitation, and 1:400 for both immunofluorescence and flow cytometry.