This rabbit monoclonal antibody is designed for the detection of endogenous levels of total U2AF2 protein, including cleavage products at 43 kDa and 46 kDa. U2AF2, the 65 kDa large subunit of the U2 auxiliary factor, plays a critical role in pre-mRNA splicing by recognizing the polypyrimidine tract upstream of the 3' splice site. This antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val275 of human U2AF2 protein.
- Validated for Western blotting applications
- Recognizes endogenous total protein
- Species reactivity includes human, mouse, rat, and monkey
- Host species is rabbit
- Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, and <0.02% sodium azide