5-Ethynyl-2'-deoxyuridine (5-EdU) is a thymidine analog that can be incorporated into replicating DNA for detection of cell proliferation. 5-EdU can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle through click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. 5-EdU has an alkyne handle that can be detected using azide-containing fluorescent probes through a highly efficient click chemistry reaction. In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. 5-EdU-labelled cells may be isolated to determine the transcript of cells, from neural cancer or tissues that have undergone division in vitro and in vivo. Other sizes are also available. Please inqury us for quote.